2017-08-22 16:04:132025-05-27 15:16:24
locus
BSU18480
BSU_18480
geneLength
891
894
outlinks
uniprot
P14383
P0CI77
outlinks
bsu
BSU18480
BSU_18480
Gene
Coordinates
2,016,845 → 2,017,738
2,016,845 2,017,738
The protein
Catalyzed reaction/ biological activity
1-pyrroline-5-carboxylate + NADPH + H+ --> L-proline + NADP+ [pubmed|28824574]
1-pyrroline-5-carboxylate + NAD(P)H + H+ --> L-proline + NAD(P)+ [pubmed|28824574]
The protein
Paralogous protein(s)
[[protein|ProI]]
[[this]]
The protein
Structure
[PDB|5BSE] (from ''Medicago trunculata'', 36% identity)
[PDB|5BSE] (from ''Medicago truncatula'', 36% identity) [pubmed|26579138]
Biological materials
Mutant
MGNA-B083 (proH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1082 NBRP B. subtilis, Japan]
HB24914 ([[gene|proH]]::''erm''), available in [SW|John Helmann]'s and [SW|Jörg Stülke]'s labs
MGNA-B083 (proH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1082 NBRP B. subtilis, Japan]
BKE18480 ([[gene|proH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCACACGTTTAAAAT, downstream forward: _UP4_GCGCCGCTATCAGGAGTGAT
BKK18480 ([[gene|proH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCACACGTTTAAAAT, downstream forward: _UP4_GCGCCGCTATCAGGAGTGAT
References
Original publications